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newborn calf serum  (R&D Systems)


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    Structured Review

    R&D Systems newborn calf serum
    Newborn Calf Serum, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/newborn+calf+serum/Newborn+Calf+Serum/pm41820674-307-20-23
    Average 94 stars, based on 22 article reviews
    newborn calf serum - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Staining:

    Article Title: A robust pipeline for high-content, high-throughput immunophenotyping reveals age- and genetics-dependent changes in blood leukocytes
    Article Snippet: .. DS-Cav1 tetramer production and staining was performed according to Crank MC et al. Staining reagents except Live/Dead UV Blue viability dye were mixed into Brilliant stain buffer plus (BD Biosciences, final dilution 1:5) and volume was adjusted with staining buffer which consisted of RPMI w/o Phenol Red (Thermo Fisher Scientific) containing 4% heat-inactivated Newborn Calf Serum (R&D Systems). .. For the intracellular antibody mix from the T cell effector staining (ICS) panel, staining reagents were diluted into double distilled H 2 O (ddH 2 O) containing Brilliant stain buffer plus (final dilution 1:5) and Wash/Perm buffer 10x from the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific, final dilution 1:10).

    Article Title: A robust pipeline for high-content, high-throughput immunophenotyping reveals age- and genetics-dependent changes in blood leukocytes.
    Article Snippet: .. DS-Cav1 tetramer production and staining was performed according to Crank MC et al.51 Staining reagents except Live/Dead UV Blue viability dye were mixed into Brilliant stain buffer plus (BD Biosciences, final dilution 1:5) and volume was adjusted with staining buffer which consisted of RPMI w/o Phenol Red (Thermo Fisher Scientific) containing 4% heat-inactivated Newborn Calf Serum (R&D Systems). .. For the intracellular antibody mix from the T cell effector staining (ICS) panel, staining reagents were diluted into double distilled H2O (ddH2O) containing Brilliant stain buffer plus (final dilution 1:5) and Wash/Perm buffer 10x from the eBioscience Foxp3/ Transcription Factor Staining Buffer Set (Thermo Fisher Scientific, final dilution 1:10).

    Article Title: A structure-based mRNA vaccine for Nipah virus in healthy adults: a phase 1 trial.
    Article Snippet: .. Assessment of B cell responses by B cell probe binding Cryopreserved PBMCs were thawed, washed briefly with phenol-free RPMI/4% heat-inactivated newborn calf serum (R&D Systems), and incubated with an aqua live/dead fixable dead cell stain kit (Thermo Fisher Scientific) for 20 min at room temperature. .. Cells were stained with the following antibodies (all monoclonal unless indicated) for 20 min at room temperature: IgD FITC (1:160 dilution) (goat polyclonal, Southern Biotech), IgM PerCP-Cy5.5 (1:20 dilution) (clone G20-127, BD Biosciences), IgA Dylight 405 (1:80 dilution) (goat polyclonal, Jackson Immunoresearch), CD20 BV570 (1:40 dilution) (clone 2H7, BioLegend), CD27 BV650 (1:20 dilution) (clone O323, BioLegend), CD14 BV785 (1:80 dilution) (clone M5E2, BioLegend), CD16 BUV496 (1:80 dilution) (clone 3G8, BD Biosciences), CD4 BUV737 (1:320 dilution) (clone SK3, BD Biosciences), CD19 APC (1:80 dilution) (clone J3-119, Beckman), IgG Alexa 700 (1:20 dilution) (clone G18-145, BD Biosciences), CD3 APC-Cy7 (1:40 dilution) (clone SP34-2, BD Biosciences), CD38 PE (1:160 dilution) (clone OKT10, Caprico Biotechnologies), CD21 PE-Cy5 (1:40 dilution) (clone B-ly4, BD Biosciences) and CXCR5 PE-Cy7 (1:40 dilution) (clone MU5UBEE, Thermo Fisher Scientific).

    Article Title: A structure-based mRNA vaccine for Nipah virus in healthy adults: a phase 1 trial
    Article Snippet: .. Cryopreserved PBMCs were thawed, washed briefly with phenol-free RPMI/4% heat-inactivated newborn calf serum (R&D Systems), and incubated with an aqua live/dead fixable dead cell stain kit (Thermo Fisher Scientific) for 20 min at room temperature. .. Cells were stained with the following antibodies (all monoclonal unless indicated) for 20 min at room temperature: IgD FITC (1:160 dilution) (goat polyclonal, Southern Biotech), IgM PerCP-Cy5.5 (1:20 dilution) (clone G20-127, BD Biosciences), IgA Dylight 405 (1:80 dilution) (goat polyclonal, Jackson Immunoresearch), CD20 BV570 (1:40 dilution) (clone 2H7, BioLegend), CD27 BV650 (1:20 dilution) (clone O323, BioLegend), CD14 BV785 (1:80 dilution) (clone M5E2, BioLegend), CD16 BUV496 (1:80 dilution) (clone 3G8, BD Biosciences), CD4 BUV737 (1:320 dilution) (clone SK3, BD Biosciences), CD19 APC (1:80 dilution) (clone J3-119, Beckman), IgG Alexa 700 (1:20 dilution) (clone G18-145, BD Biosciences), CD3 APC-Cy7 (1:40 dilution) (clone SP34-2, BD Biosciences), CD38 PE (1:160 dilution) (clone OKT10, Caprico Biotechnologies), CD21 PE-Cy5 (1:40 dilution) (clone B-ly4, BD Biosciences) and CXCR5 PE-Cy7 (1:40 dilution) (clone MU5UBEE, Thermo Fisher Scientific).

    Cell Culture:

    Article Title: Human papillomaviruses sensitize cells to DNA damage induced apoptosis by targeting the innate immune sensor cGAS
    Article Snippet: .. NIH-3T3 J2 fibroblasts were cultured and passaged in DMEM (Dulbecco’s Modified Eagle Medium, Gibco) supplemented with 10% newborn calf serum (R&D Systems) and 1% Penicillin-Streptomycin (Gibco). ..

    Article Title: Regulation of the formin INF2 by actin monomers and calcium-calmodulin
    Article Snippet: Oligonucleotides used for siRNA mediated gene silencing (Integrated DNA Technologies): CAP1 (exon 12): 5’-GTCAGTGCCAAATCTTCC-3’, CAP2 (3’ UTR): 5’-CTTTGAGAATCTAAGATG-3’. .. Human osteosarcoma U2-OS cells (ATCC HTB-96) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Corning, 10-013-CV) supplemented with 10% newborn calf serum (R&D Systems; S11250). .. Human cervical cancer HeLa cells (ATCC CCL-2) were cultured in DMEM supplemented with 10% fetal bovine serum (Sigma-Aldrich; F4135).

    Article Title: Regulation of the formin INF2 by actin monomers and calcium-calmodulin
    Article Snippet: Oligonucleotides used for siRNA mediated gene silencing (Integrated DNA Technologies): CAP1 (exon 12): 5’-GTCAGTGCCAAATCTTCC-3’, CAP2 (3’ UTR): 5’-CTTTGAGAATCTAAGATG-3’. .. Human osteosarcoma U2-OS cells (ATCC HTB-96) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Corning, 10-013-CV) supplemented with 10% newborn calf serum (R&D Systems; S11250). .. Human cervical cancer HeLa cells (ATCC CCL-2) were cultured in DMEM supplemented with 10% fetal bovine serum (Sigma-Aldrich; F4135).

    Article Title: Regulation of the formin INF2 by actin monomers and calcium/calmodulin.
    Article Snippet: In response to increased intracellular calcium, the formin INF2 polymerizes 20–30% of the total cellular actin pool within 30 s, suggesting robust regulation.. INF2 regulation requires an autoinhibitory interaction between the N-terminal diaphanous inhibitory domain (DID) and the C-terminal diaphanous autoregulatory domain (DAD).. DID mutations are dominantly linked to two human diseases and constitutively activate INF2.

    Modification:

    Article Title: Human papillomaviruses sensitize cells to DNA damage induced apoptosis by targeting the innate immune sensor cGAS
    Article Snippet: .. NIH-3T3 J2 fibroblasts were cultured and passaged in DMEM (Dulbecco’s Modified Eagle Medium, Gibco) supplemented with 10% newborn calf serum (R&D Systems) and 1% Penicillin-Streptomycin (Gibco). ..

    Article Title: Regulation of the formin INF2 by actin monomers and calcium-calmodulin
    Article Snippet: Oligonucleotides used for siRNA mediated gene silencing (Integrated DNA Technologies): CAP1 (exon 12): 5’-GTCAGTGCCAAATCTTCC-3’, CAP2 (3’ UTR): 5’-CTTTGAGAATCTAAGATG-3’. .. Human osteosarcoma U2-OS cells (ATCC HTB-96) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Corning, 10-013-CV) supplemented with 10% newborn calf serum (R&D Systems; S11250). .. Human cervical cancer HeLa cells (ATCC CCL-2) were cultured in DMEM supplemented with 10% fetal bovine serum (Sigma-Aldrich; F4135).

    Article Title: Regulation of the formin INF2 by actin monomers and calcium-calmodulin
    Article Snippet: Oligonucleotides used for siRNA mediated gene silencing (Integrated DNA Technologies): CAP1 (exon 12): 5’-GTCAGTGCCAAATCTTCC-3’, CAP2 (3’ UTR): 5’-CTTTGAGAATCTAAGATG-3’. .. Human osteosarcoma U2-OS cells (ATCC HTB-96) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Corning, 10-013-CV) supplemented with 10% newborn calf serum (R&D Systems; S11250). .. Human cervical cancer HeLa cells (ATCC CCL-2) were cultured in DMEM supplemented with 10% fetal bovine serum (Sigma-Aldrich; F4135).

    Article Title: Regulation of the formin INF2 by actin monomers and calcium/calmodulin.
    Article Snippet: In response to increased intracellular calcium, the formin INF2 polymerizes 20–30% of the total cellular actin pool within 30 s, suggesting robust regulation.. INF2 regulation requires an autoinhibitory interaction between the N-terminal diaphanous inhibitory domain (DID) and the C-terminal diaphanous autoregulatory domain (DAD).. DID mutations are dominantly linked to two human diseases and constitutively activate INF2.

    Binding Assay:

    Article Title: A structure-based mRNA vaccine for Nipah virus in healthy adults: a phase 1 trial.
    Article Snippet: .. Assessment of B cell responses by B cell probe binding Cryopreserved PBMCs were thawed, washed briefly with phenol-free RPMI/4% heat-inactivated newborn calf serum (R&D Systems), and incubated with an aqua live/dead fixable dead cell stain kit (Thermo Fisher Scientific) for 20 min at room temperature. .. Cells were stained with the following antibodies (all monoclonal unless indicated) for 20 min at room temperature: IgD FITC (1:160 dilution) (goat polyclonal, Southern Biotech), IgM PerCP-Cy5.5 (1:20 dilution) (clone G20-127, BD Biosciences), IgA Dylight 405 (1:80 dilution) (goat polyclonal, Jackson Immunoresearch), CD20 BV570 (1:40 dilution) (clone 2H7, BioLegend), CD27 BV650 (1:20 dilution) (clone O323, BioLegend), CD14 BV785 (1:80 dilution) (clone M5E2, BioLegend), CD16 BUV496 (1:80 dilution) (clone 3G8, BD Biosciences), CD4 BUV737 (1:320 dilution) (clone SK3, BD Biosciences), CD19 APC (1:80 dilution) (clone J3-119, Beckman), IgG Alexa 700 (1:20 dilution) (clone G18-145, BD Biosciences), CD3 APC-Cy7 (1:40 dilution) (clone SP34-2, BD Biosciences), CD38 PE (1:160 dilution) (clone OKT10, Caprico Biotechnologies), CD21 PE-Cy5 (1:40 dilution) (clone B-ly4, BD Biosciences) and CXCR5 PE-Cy7 (1:40 dilution) (clone MU5UBEE, Thermo Fisher Scientific).

    Incubation:

    Article Title: A structure-based mRNA vaccine for Nipah virus in healthy adults: a phase 1 trial.
    Article Snippet: .. Assessment of B cell responses by B cell probe binding Cryopreserved PBMCs were thawed, washed briefly with phenol-free RPMI/4% heat-inactivated newborn calf serum (R&D Systems), and incubated with an aqua live/dead fixable dead cell stain kit (Thermo Fisher Scientific) for 20 min at room temperature. .. Cells were stained with the following antibodies (all monoclonal unless indicated) for 20 min at room temperature: IgD FITC (1:160 dilution) (goat polyclonal, Southern Biotech), IgM PerCP-Cy5.5 (1:20 dilution) (clone G20-127, BD Biosciences), IgA Dylight 405 (1:80 dilution) (goat polyclonal, Jackson Immunoresearch), CD20 BV570 (1:40 dilution) (clone 2H7, BioLegend), CD27 BV650 (1:20 dilution) (clone O323, BioLegend), CD14 BV785 (1:80 dilution) (clone M5E2, BioLegend), CD16 BUV496 (1:80 dilution) (clone 3G8, BD Biosciences), CD4 BUV737 (1:320 dilution) (clone SK3, BD Biosciences), CD19 APC (1:80 dilution) (clone J3-119, Beckman), IgG Alexa 700 (1:20 dilution) (clone G18-145, BD Biosciences), CD3 APC-Cy7 (1:40 dilution) (clone SP34-2, BD Biosciences), CD38 PE (1:160 dilution) (clone OKT10, Caprico Biotechnologies), CD21 PE-Cy5 (1:40 dilution) (clone B-ly4, BD Biosciences) and CXCR5 PE-Cy7 (1:40 dilution) (clone MU5UBEE, Thermo Fisher Scientific).

    Article Title: A structure-based mRNA vaccine for Nipah virus in healthy adults: a phase 1 trial
    Article Snippet: .. Cryopreserved PBMCs were thawed, washed briefly with phenol-free RPMI/4% heat-inactivated newborn calf serum (R&D Systems), and incubated with an aqua live/dead fixable dead cell stain kit (Thermo Fisher Scientific) for 20 min at room temperature. .. Cells were stained with the following antibodies (all monoclonal unless indicated) for 20 min at room temperature: IgD FITC (1:160 dilution) (goat polyclonal, Southern Biotech), IgM PerCP-Cy5.5 (1:20 dilution) (clone G20-127, BD Biosciences), IgA Dylight 405 (1:80 dilution) (goat polyclonal, Jackson Immunoresearch), CD20 BV570 (1:40 dilution) (clone 2H7, BioLegend), CD27 BV650 (1:20 dilution) (clone O323, BioLegend), CD14 BV785 (1:80 dilution) (clone M5E2, BioLegend), CD16 BUV496 (1:80 dilution) (clone 3G8, BD Biosciences), CD4 BUV737 (1:320 dilution) (clone SK3, BD Biosciences), CD19 APC (1:80 dilution) (clone J3-119, Beckman), IgG Alexa 700 (1:20 dilution) (clone G18-145, BD Biosciences), CD3 APC-Cy7 (1:40 dilution) (clone SP34-2, BD Biosciences), CD38 PE (1:160 dilution) (clone OKT10, Caprico Biotechnologies), CD21 PE-Cy5 (1:40 dilution) (clone B-ly4, BD Biosciences) and CXCR5 PE-Cy7 (1:40 dilution) (clone MU5UBEE, Thermo Fisher Scientific).



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